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Buying Frozen Dog Sperm ((TOP))


We are an AKC approved permanent storage facility. Frozen semen is stored within our facility in a specialized insulated container called a dewar that contains liquid nitrogen. The frozen semen is held in liquid nitrogen vapors at a temperature of -196 degrees Centigrade or approximately 300 degrees below zero Fahrenheit. This temperature is maintained and keeps the semen viable indefinitely. Since the container does not require electricity, there is no fear of accidental thawing due to a power failure.




buying frozen dog sperm



Current fees are approximately $445 for the freeze and $94 per year storage. If you have an AKC or UKC breed, these registration bodies require a DNA number be on file and will cost approximately $40-45 to process. A breeding with frozen semen entails ovulation timing and a surgical insemination is usually preferred. Costs vary and can be discussed individually.


Canine Scanning are here to help; when your bitch enters her season, our team will immediately begin to search for suitable stud dogs. Our bank of dog sperm means we can find a stud dog to match your budget and your requirements, making your life a whole lot easier.


All of our chilled dog semen samples are fully analysed for volume, motility and morphology of the canine spermatozoa. That means we can guarantee a high success rate, giving you peace of mind that when you buy chilled semen from Canine Scanning you investment will result in successful breeding.


Canine Scanning can now offer a complete chilled dog semen shipping service, which enables us to offer fully tested and prepared dog semen for sale from our own pedigree stud dogs. We can prepare and ship chilled dog semen anywhere worldwide within 48-hours from our Liverpool-based canine sperm bank.


With the advances in scientific knowledge and the technology to provide verification of parentage, registries are now being held to a higher standard. It used to be that the owner of the bitch and the stud were the only two individuals supervising a breeding, but with the use of frozen and fresh chilled semen, the number of individuals handling the breeding has multiplied, i.e. one to collect, a technician or two to process the semen and freeze, one to retrieve and reactivate, and one to inseminate. The more individuals involved in this complex process the more room for mistakes to be made. A Registration Office would be negligent if additional verification was not required in the registration of litters or individuals using FROZEN SEMEN and FRESH CHILLED SEMEN.


The ADBA Inc. has DNA profiling services, so you can visit our website or call our office to order Collection Kits. Dog must be ADBA registered prior to being profiled. The fee for these kits are $6.00 for the cheek swab or for a Blood FTA card, per dog being profiled.Forms for use of frozen semen can also be printed off our website at ADBADOG.COM.


3. If the dogs produced are to be single dog registered, the breeder will need to file an AIFS form with the ADBA for the breeding with the fee of $23.00. ADBA will record and keep this on file. The single application forms from this frozen semen breeding will then be accepted.


Q: What is the transfer of ownership?A: A transfer of ownership of frozen semen (TRSF) form will need to be filled and signed by the owner of the semen, giving the new owner the rights to a specific number of breeding straws. This will need to be recorded with ADBA by the new owner. The fee for recording is $15.00.


Hello ADBA,Q: I have semen that has been frozen. The dog has not been transferred into my name and he is now deceased. I did not know about the frozen semen policy. What do I have to do now in order to get the semen recognized by the ADBA so I can use it?A: You need to have the dog transferred into your ownership. Make sure the date of sale is accurate and filled out. You need to have owned the dog at the time of collection.Send in a record of where the semen has been stored and the quantity of semen stored. You will need to have a DNA profile done on the dog. Since the dog is deceased, to obtain a DNA profile, a straw will need to be thawed and sent to the lab to obtain the DNA profile. If you do not want to waste a straw, you can have the vet save 2 drops at the time you are inseminating a female.


Many factors contribute to the likelihood of pregnancy when using artificial insemination, including type of semen (fresh or frozen), semen quality and quantity, age and fertility of both the stud dog and bitch, site of semen deposition (intrauterine or vaginal), and time of insemination.


The intrauterine insemination technique that we recommend when using frozen-thawed semen or low numbers of potentially compromised fresh or chilled semen is transcervical insemination, where semen is deposited directly into the uterus to maximize the chance of pregnancy.


When using fresh or chilled semen with adequate sperm numbers and sperm quality, deposition of semen into the very end of the vagina can result in pregnancy and litter sizes equivalent to natural mating.


No matter what type of semen (fresh, fresh-chilled, or frozen) or the method of artificial insemination that is used to deposit the semen, careful and skillful handling of the semen is essential. Furthermore, assessment of a sample of semen by a qualified and knowledgeable professional prior to insemination plays a critical role in the maximization of pregnancy rates.


This is a sample timeline for transcervical insemination using frozen semen. Because the lifespan of frozen semen once thawed is limited, timing of insemination is critical. The process using fresh or fresh-chilled semen is similar, and this timeline reflects the most regimented version of the artificial insemination process.


Is a dose a breeding? Is a straw a dose? How many straws in a dose? How many sperm in a dose? These are often questions we hear from our clients when purchasing or using frozen semen. There is so much variability in the format of doses sold throughout the world that it can be confusing at times. To ensure you have the best opportunity of success when breeding your mare it is important to understand all the elements that determine an adequate dose of frozen semen. Here we review frequently asked questions that relate to what constitutes a dose of frozen semen.


There are currently no universally accepted standards for the production of quality semen by AI centers in North America, see our blog article Should the US Adopt Stricter Controls On Cooled and Frozen Semen Production Facilities. However, it is generally accepted that a dose of frozen semen should contain a minimum of 200 million progressively motile sperm. A European based organization called the World Breeding Federation of Sport Horses (WBFSH) several years ago published recommended guidelines for semen production and quality standards for its member breeding stations; the details of these guidelines can be found here.


However, one must remember that although motility is an indicator of relative cell health, fertilization is a complex process that requires numerous functional attributes of both sperm and egg. Therefore the true fertility of any frozen semen can only be determined by properly timed insemination of reproductively healthy mares.


A breeding dose of 8 x 0.5mL straws consists of a total of 800 million sperm and a post-thaw progressive motility of 35%.The dose volume is 4mL (8 x 0.5mL straws)The sperm concentration is 200 million/mL. We know this because the total sperm in the dose is 800 million, so 800 divided by the volume of 4mL = 200 million sperm/mL


All too often you may not receive information on the sperm concentration or on the number of PMS in the dose, and sometimes the motility quoted is the average motility across several lots of semen, not for that collection date in particular. It is therefore important to ask the appropriate questions when purchasing frozen semen, so you can determine if the dose you are about to purchase contains an acceptable amount of sperm. See our blog Questions Every Mare Owner Should Ask Before Breeding with Frozen Semen?


A breeding dose prepared by an SBS Affiliated Laboratory is standardized to comprise of eight 0.5mL straws. Semen is frozen at a concentration of 200-250 million/mL, resulting in a total of 800 million to 1 billion sperm in a final dose volume of 4mL. Given our minimum recommended post-thaw motility for commercial distribution of 30% progressive, each breeding dose therefore also exceeds the industry recommended minimum of 200 million progressively motile sperm per dose after thawing.


At SBS we determine concentration after centrifugation, by either hemocytometer or nucleocounter, and then dilute the sperm to 200-250 million/mL in freezing extender before filing the straws. We perform a post-thaw evaluation on 2 pooled straws from every ejaculate frozen. After thawing, the semen is cultured to confirm the absence of bacterial growth and sperm motility is evaluated after the thawed semen is diluted in extender and incubated at 37C for 30 minutes.


Straws are the typical packaging unit for frozen semen, it is also possible although unusual, to receive frozen semen in pellets and packets. The most common straw size in use today is the 0.5mL straw, although you may occasionally come across 0.25mL straws. In the past semen was frozen in 4mL or 5mL straws, also known as macro-tubes. In the last 10 years, SBS has opted to use 0.5mL straws because a more consistent freezing rate within the straw can be achieved, as the straw diameter is considerably less than that of the macro straw. When the freezing rate of all of the sperm within the straw is consistent, then the overall quality of the semen is maximized once they are thawed and ready for insemination. Another benefit achieved from using 0.5 mL straws, is that they can be used with mechanical filling and sealing machines which ensure consistent filling of straws, a secure seal and a reduced processing time. Furthermore, 0.5mL straws lend themselves to several different methods of convenient storage in either large or small goblets or on canes. Plus post-thaw evaluation of 1 or 2 (0.5mL) straws from each batch represents only 1/8 to 1/4 of a breeding dose, whereas for macrotubes one straw often constitutes one dose, so when performing a post-thaw evaluation on one straw, one dose of semen is lost. 041b061a72


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